Team 3: Epithelial pathophysiology, stem cells and tissue repair (team leader: Daniel Aberdam)

From left to right:

Giulia Thomson, Andrea Trentin, Léna Ronjat, Livia Camargo, Linda Sangari, Isabelle Petit, Alizée Le Riche, Daniel Aberdam, Edith Aberdam, Frédéric Guaddachi, Sébastien Jauliac et Lauriane Roux.

Main projects:  (have a look at the team Web site)

(main publications below)

1. Epithelial commitment, differentiation and aging (Ayelet Levy; Isabelle Petit)  
Our interest is mainly focused on the molecular events underlying the cross-talk between stem cells during skin development, homeostasis, tissue repair and aging. For these studies, we are using pluripotent and somatic stem cells, functional genomic assays, co-culture, 3D organogenesis and epigenetic modulation. 

2. NFAT function and regulation in epithelial pathophysiology (Sébastien Jauliac) 
We are studying the regulation and function of NFAT factors in epithelial homeostasis and physiopathology. We have demonstrated for the first time the implication of the NFAT pathway in the regulation epithelial cells motility in breast carcinoma and highlighted the NFAT factors as critical components of epithelial cell fate. 

3.  Ectodermal dysplasia, Aniridia and corneal cell/gene therapy (Edith Aberdam)
Our goal is to provide alternative tools for acquired (wounds, burns) and hereditary (aniridia and ectodermal dysplasia) corneal opacification. We have recently designed a protocol to efficiently produce corneal epithelial cells from pluripotent stem cells derived from patient samples (hair follicles and skin biopsies). This novel methodology will be optimized to develop a "ready-to-use" transplantable cornea but also a cellular model for toxicity assays of cosmetic products and pharmacological tests of drugs. 

Main Publications: 


Daniel Aberdam and Isabelle Petit (Sébastien Jauliac, see below):


2016
1. Erbani J, Aberdam D, Larghero J, Vanneaux V. Pluripotent Stem Cells and Other Innovative Strategies for the Treatment of Ocular Surface Diseases. Stem Cell Rev. 2016 Jan 16. [Epub ahead of print] . 

2. Nagosa S., Leesch F., Putin D, Aberdam E., Lange-Amitai A., Aberdam D*. and Shalom-Feuerstein R*. microRNA-184 regulates a switch from epidermal stemness to cell differentiation. Embo J., submitted. (*) co-senior authors.

2015

1. Moussaieff A, Rouleau M, Kitsberg D, Cohen M, Levy G, Barasch D, Nemirovski A, Shen-Orr S, Laevsky I, Amit M, Bomze D, Elena-Herrmann B, Scherf T, Nissim-Rafinia M, Kempa S, Itskovitz-Eldor J, Meshorer E, Nahmias Y* and Aberdam D*. Glycolysis-mediated Changes in Acetyl-coA and Histone Acetylation Control the Early Differentiation of Embryonic Stem Cells. Cell Metab. 21(3):392-402. * co last senior authors. 


Preview by Dr. G. Daley: Shyh-Chang N, Daley GQ. Metabolic switches linked to pluripotency and embryonic stem cell differentiation. Cell Metab. (2015) 21(3):349-50.


2. Moussaieff A, Kogan NM, Aberdam D. Energy Metabolites: Key Mediators of the Epigenetic State of Pluripotency. Stem Cells. 33(8):2374-80.


3. Antonini D, Sirico A, Aberdam E, Ambrosio R, Campanile C, Fagoonee F, Aberdam D, Brissette JL and Missero C. 2015. A composite enhancer regulates p63 gene expression in epidermal morphogenesis and in keratinocyte differentiation by multiple mechanisms.  Nucl Ac Res. 43(2):862-74.


4. Warshauer E, Samuelov L, Sarig O, Vodo D, Bindereif A, Kanaan M, Gat U, Fuchs-Telem D, Shomron N, Farberov L, Pasmanik-Chor M, Nardini G, Winkler E, Meilik B, Petit I, Aberdam D, Paus R, Sprecher Eli and Nousbeck J. (2015) RBM28, a protein deficient in ANE syndrome, regulates hair follicle growth via miR-203 and p63. Exp. Dermatol., 24(8):618-22.

5. Levy A, Petit I and Aberdam D. (2015) Pluripotent stem cells as a cellular model for skin: relevance for physiopathology, cell/gene therapy and drug screening. Eur. J. Dermatol, Apr;25 Suppl 1:12-7.
6. Aberdam D. [Metabolic switch in pluripotent stem cells: identification of a novel pathway]. Med Sci (Paris). 2015 Jun-Jul;31(6-7):581-3. 

 

2014

1. Sabapathy K, Nagakawara A, Aberdam D. The 6th International p63/p73 Workshop: the C(ancer) and D(evelopmental) roles of p63 and p73. Cell Death Differ. 2014 21, 1340-2.


2. Tattikota SG, Rathjen T, McAnulty SJ, Wessels HH, Akerman I, van de Bunt M, Hausser J, Esguerra JL, Musahl A, Pandey AK, You X, Chen W, Herrera PL, Johnson PR, O'Carroll D, Eliasson L, Zavolan M, Gloyn AL, Ferrer J, Shalom-Feuerstein R,  Aberdam D, Poy MN. Argonaute2 Mediates Compensatory Expansion of the Pancreatic β Cell. Cell Metab. 19(1):122-34.


3. Wolchinsky Z, Shivtiel S, Kouwenhoven EN, Putin D, Sprecher E, Zhou H, Rouleau M, Aberdam D. Angiomodulin is required for cardiogenesis of embryonic stem cells  and is maintained by a feedback loop network of p63 and Activin-A. Stem Cell Res. 2014 Jan;12(1):49-59.


4. T. Goldsmith, O. Eytan, L. Magal, M. Solomon, S. Israeli, E. Warshauer, M. Grafi-Cohen, D. Aberdam, H. van Bokhoven, J.H. Zhou, O. Sarig, E. Sprecher,  J. Nousbeck. A novel mutation inTP63 causing a mild ectodermal dysplasia phenotype. J. Invest. dermatol. 134, 2277-80.


5. Freije A., Ceballos L., Cagigas M., Molinuevo R., Alonso P., Rodriguez R., Menendez P., Aberdam D., De Diego E. and Gandarillas A. Inactivation of p53 in human keratinocytes leads to squamous differentiation and shedding via replication stress and mitotic slippage. Cell Report. 9(4):1349-1360.


6. Leshansky L, Aberdam D, Itskovitz-Eldor J, Berrih-Aknin S. Human Embryonic Stem Cells Prevent T-Cell Activation by Suppressing Dendritic Cells Function via  TGF-Beta Signaling Pathway. Stem Cells. 2014 Dec;32(12):3137-49.


2013

-1.  Shalom-Feuerstein R, Serror L, Aberdam E, Müller FJ, van Bokhoven H, Wiman KG, Zhou H, Petit I* and Aberdam D*. (2013) Impaired epithelial differentiation of induced pluripotent stem cells from EEC patients is rescued by APR-246/PRIMA-1MET. Proc Natl Acad Sci U S A,  110(6):2152-2156. * co last authors


-2. Robicsek, O, Karry R., Petit I, Salman Kesner N., Müller FJ., Klein E., Aberdam D. and D. Ben Shachar. (2013) Abnormal neuronal differentiation and mitochondrial dysfunction in hair follicle-derived induced pluripotent stem cells of schizophrenia patients. Molecular Psychiatry, 18, 1067-76.


-3. Zhou H, Aberdam D. A step closer toward therapies for p63-related disorders. Rare Dis.2013 Mar 12;1:e24247.


2012

-1. Petit I, Salman Kesner N, Karry R, Robicsek O,  Aberdam E, Müller FJ, Aberdam D* and Ben Shachar D*. Induced Pluripotent Stem Cells from hair follicles as a novel cellular model for neurodevelopmental disorders. Stem cell Res. Jan;8(1):134-40. (*co-last authors) pdf


-2. Shalom-Feuerstein R, Serror L, De La Forest Divonne S, Petit I, Aberdam E, Odile Damour O, Vigouroux C, Solomon A, Camargo L, Gagioli C, Itskovitz-Eldor J, Ahmad S and Aberdam D (2012) Pluripotent stem cell model reveals essential roles for miR-450b-5p and miR-184 in embryonic corneal lineage specification. Stem cells 30(5):898-909. pdf


-3. Fuchs G, Shema E, Vesterman R, Kotler E,  Wolchinsky Z, Wilder S, Golomb L, Pribluda A, Zhang F, Haj-Yahya M, Feldmesser E, Brik A, Yu X, Hanna J, Aberdam D, Domany E and Oren M. (2012) RNF20 and USP44 regulate stem cell differentiation by modulating H2B monoubiquitylation. Mol. Cell, 46(5):662-73.


-4.  Hof-Nahor I, Leshansky L, Shivtiel S, Eldor L, Aberdam D, Itskovitz-Eldor J, Berrih-Aknin S. Human mesenchymal stem cells shift CD8+ T cells towards a suppressive phenotype by inducing tolerogenic monocytes. J Cell Sci. 2012 Jul 5. [Epub ahead of print]


-5. Amelio I, Lena AM, Viticchiè G, Shalom-Feuerstein R, Terrinoni A, Dinsdale D,  Russo G, Fortunato C, Bonanno E, Spagnoli LG, Aberdam D, Knight RA, Candi E, Melino G. miR-24 triggers epidermal differentiation by controlling actin adhesion and cell migration. J Cell Biol.199(2): 347-63. pdf




2011

-1. Rouleau M, Medawar A, Hamon L, Shivtiel S, Wolchinsky Z, Zhou H, De Rosa L, Candi E, Divonne de la Forest S, Mikkola ML, van Bokhoven H, Missero C, Melino G, Puceat M and Aberdam D. TAp63 is important for cardiac differentiation of embryonic stem cells and heart development. Stem cells 29(11):1672-83.  pdf. 


-2. Shalom-Feuerstein R., Lena AM., Zhou H., De La Forest Divonne S., Van Bokhoven H., Candi E., Melino G. and  Aberdam D. DNp63 is an ectodermal gatekeeper of epidermal morphogenesis. Cell Death Diff.18:887-96. pdf


-3. V Marcel, I Petit, F Murray-Zmijewski, T Goullet de Rugy, K Fernandes, V Meuray, A Diot, DP. Lane, D Aberdam and JC Bourdon. Diverse p63 and p73 isoforms regulate Δ133p53 expression through modulation of the internal TP53 promoter activity, Cell Death Diff.19:816-26. pdf


-4. Paris M, Rouleau M, Pucéat M and Aberdam D. Regulation of skin aging and heart development by TAp63. Cell Death Diff. 19(2):186-93. pdf


2009

-1. Hooper AT, Butler JM, Nolan DJ, Kranz A, Iida K, Kobayashi M, Kopp HG, Shido K, Petit I, Yanger K, James D, Witte L, Zhu Z, Wu Y, Pytowski B, Rosenwaks Z, Mittal V, Sato TN, Rafii S. Engraftment and reconstitution of hematopoiesis is dependent on VEGFR2-mediated regeneration of sinusoidal endothelial cells. Cell Stem Cell. 2009 Mar 6;4(3):263-74. 


-2. Vagima Y, Avigdor A, Goichberg P, Shivtiel S, Tesio M, Kalinkovich A, Golan K, Dar A, Kollet O, Petit I, Perl O, Rosenthal E, Resnick I, Hardan I, Gellman YN, Naor D, Nagler A, Lapidot T. MT1-MMP and RECK are involved in human CD34+ progenitor cell retention, egress, and mobilization. J Clin Invest. 2009 Mar;119(3):492-503.  


2008

-1. Aberdam E, Barak E, Rouleau M, De Laforest S, Berrih-Aknin S, Suter DM, Krause KH, Amit M, Itskovitz-Eldor J, and Aberdam D. A pure ectodermal cell population derived from human embryonic stem cells. Stem cells 26:440-444. pdf 


-2. Medawar A, , Rostagno P., De Laforest Divonne S, Gambaro K., Rouleau M. and Aberdam D. DNp63 is essential for epidermal commitment of embryonic stem cells. Plos One, 3:e3441. pdf


-3. Aberdam D., Candi E., Knight R.A. and Melino G. miR, “stemness” and skin. Trends Biochem. Sci. 33(12):583-91. pdf


-4. Shivtiel S, Kollet O, Lapid K, Schajnovitz A, Goichberg P, Kalinkovich A, Shezen E, Tesio M, Netzer N, Petit I, Sharir A, Lapidot T. CD45 regulates retention, motility, and numbers of hematopoietic progenitors, and affects osteoclast remodeling of metaphyseal trabecules. J Exp Med. 2008 Sep 29;205(10):2381-95.


-5. Petit I, Karajannis MA, Vincent L, Young L, Butler J, Hooper AT, Shido K, Steller H, Chaplin DJ, Feldman E, Rafii S. The microtubule-targeting agent CA4P regresses leukemic xenografts by disrupting interaction with vascular cells and mitochondrial-dependent cell death. Blood. 2008 Feb 15;111(4):1951-61.


2007

-1. Aberdam D, Gambaro K, Aberdam E, Rostagno P, de la Forest Divonne S and Rouleau M. Role of p63 during epidermal commitment of embryonic stem cells. Cell cycle 6:291-4.


-2.  Hooper AT, Butler J, Petit I, Rafii S. Does N-cadherin regulate interaction of hematopoietic stem cells with their niches? Cell Stem Cell. 2007 Aug 16;1(2):127-9.


-3. Petit I, Jin D, Rafii S. The SDF-1-CXCR4 signaling pathway: a molecular hub modulating neo-angiogenesis. Trends Immunol. 2007 Jul;28(7):299-307.

 

2006

-1. Gambaro K, Aberdam E, Virolle T, Aberdam D and Rouleau M. BMP-4 induces the Smad-dependent apoptotic cell death of mouse embryonic stem cell-derived neural progenitors. Cell Death & Diff. 13, 1075-1087.

 

2005

-1. Thyss R, Virolle-de Thillot V, Imbert V, Peyron JF, Aberdam D and Virolle T. NF-KB/Egr-1/Gadd45 are sequentially activated upon UVB irradiation to mediate epidermal cell death. Embo J. 24:128-137.


-2. Tavor S, Petit I, Porozov S, Goichberg P, Avigdor A, Sagiv S, Nagler A, Naparstek E, Lapidot T. Motility, proliferation, and egress to the circulation of human AML cells are elastase dependent in NOD/SCID chimeric mice. Blood. 2005 Sep 15;106(6):2120-7.


-3. Petit I, Goichberg P, Spiegel A, Peled A, Brodie C, Seger R, Nagler A, Alon R, Lapidot T. Atypical PKC-zeta regulates SDF-1-mediated migration and development of human CD34+ progenitor cells. J Clin Invest. 2005 Jan;115(1):168-76.


-4. Tavor S, Petit I, Porozov S, Avigdor A, Dar A, Leider-Trejo L, Shemtov N, Deutsch V, Naparstek E, Nagler A, Lapidot T. CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice. Cancer Res. 2005 Apr 15;64(8):2817-24. 


2003

-1.Coraux C, Hilmi C, Rouleau M, Spadafora A, Hinnrasky J, Ortonne JP, Dani C, Aberdam D. (2003) Reconstituted skin from murine embryonic stem cells. Curr Biol. 13, 849-53. pdf


2002

-1. Virolle T, Coraux C, Ferrigno O, Cailleteau L, Ortonne JP, Pognonec P, Aberdam D. Binding of USF to a non-canonical E-box following stress results in a cell-specific derepression of the lama3 gene. Nucleic Acids Res. 30, 1789-98.

 

2001

-1. Ferrigno O., Virolle T., Djabari Z., Ortonne J.P., White R.J., and Aberdam D. Transposable B2 SINE Elements Can Provide a Mobile RNA Polymerase II Promoter. Nature genetics 28, 77-81. pdf


2000

-1.Virolle T., Djabari Z., Ortonne J.P. and Aberdam D. DNA conformation driven by AP-1 triggers cell-specific expression via a strong epithelial enhancer. Embo Reports 1, 328-333.pdf


1998

-1. Virolle T., Monthouel M.N., Djabari Z., Ortonne J.P., Meneguzzi G. and Aberdam D. Three AP-1 binding sites bound by Fra-2/JunD complex cooperate for the regulation of the murine laminin a3A (lama3A) promoter activity by transforming growth factor-beta. J. Biol. Chem. 273, 17318- 17325.


1997

-1. Ferrigno O., Virolle T., Galliano M.F., Chauvin N., Ortonne J.P., Meneguzzi G. and Aberdam D. Murine laminin 5 a3A and a3B isoform chains are generated by usage of two promoters and alternative splicing. J. Biol. Chem. 272, 20502-20508.


1995

-1. Vidal F., Aberdam D., Christiano A.M., Uitto J., Ortonne J.P. and Meneguzzi G. Integrin ß4 mutations are associated with junctional epidermolysis bullosa with pyloric atresia. Nature genetics 10, 229-234.


1994

-1. Aberdam D., Galliano M.F., Vailly J., Pulkkinen L., Bonifas J., Christiano A.M., Tryggvason K., Uitto J., Epstein E.H. Jr, Ortonne J.P. and Menneguzi G. Herlitz's junctional epidermolysis bullosa is genetically linked to mutations in the gene for the g2 subunit of nicein/kalinin (laminin-5). Nature genetics 6, 299-304. pdf


1991 

-1. Aberdam D., Negreanu V., Sachs L. and Blatt C. The oncogenic potential of an activated Hox-2.4 homeobox gene in mouse fibroblasts. Mol. Cell. Biol. 11, 554-557. pdf

Sébastien Jauliac's main publications:


2014

1. Brun C, Demeaux A, Guaddachi F, Jean-Louis F, Oddos T, Bagot M, Bensussan A, Jauliac S, Michel L. T-plastin expression downstream to the calcineurin/NFAT pathway is involved in keratinocyte migration. PLoS One. 2014 Sep 16;9(9):e104700.  


2012

1: Gaudineau B, Fougère M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S.Lipocalin 2 (LCN2), the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion. J Cell Sci.,125 (19):4475-86.  

 

2: Germann S, Gratadou L, Zonta E, Dardenne E, Gaudineau B, Fougère M, Samaan S, Dutertre M, Jauliac S, Auboeuf D. Dual role of the ddx5/ddx17 RNA helicases in the control of the pro-migratory NFAT5 transcription factor. Oncogene. 31(42):4536-49.  


3: Bégué E, Jean-Louis F, Bagot M, Jauliac S, Cayuela JM, Laroche L, Parquet N, Bachelez H, Bensussan A, Courtois G, Michel L. Inducible expression and pathophysiologic functions of T-plastin in cutaneous T-cell lymphoma. Blood. 120(1):143-54. 


2010

1: Fougère M, Gaudineau B, Barbier J, Guaddachi F, Feugeas JP, Auboeuf D, Jauliac S. NFAT3 transcription factor inhibits breast cancer cell motility by targeting the Lipocalin 2 gene. Oncogene. 29(15):2292-301.  


2005

1: Yoeli-Lerner M, Yiu GK, Rabinovitz I, Erhardt P, Jauliac S, Toker A. Akt blocks breast cancer cell motility and invasion through the transcription factor NFAT. Mol Cell. 20(4):539-50.  


2002:

Jauliac S, López-Rodriguez C, Shaw LM, Brown LF, Rao A, Toker A. The role of NFAT transcription factors in integrin-mediated carcinoma invasion. Nat Cell Biol. 4(7):540-4.